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Cenci MA, Bjrklund A

Final results of a phase Ib dose-escalation study of PRI-724, a CBP/beta-catenin modulator, plus gemcitabine (GEM) in patients with advanced pancreatic adenocarcinoma (APC) as second-line therapy after FOLFIRINOX or FOLFOX

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To measure complex-specific H 2 O 2 production, the cells were incubated with 10 mM pyruvate/1 mM malate, 10 mM succinate/2 M rotenone, or 5 mM glycrol-3-phosphate/2 M rotenone diluted in MAS buffer containing 4 mM ADP and 1 nM XF PMP for 30 min at 37 C prior to the addition of Amplex Red

Beyond these direct effects on redox status, Nrf2 also modulates the expression of genes implicated in cellular defense, thereby inhibiting redox cycle reactions and diminishing GSH consumption (52)